因为种内变异、基因和物种历史不一致， Pons J，特别是在仅使用线粒体 DNA 的单个片段的情况下（ Lipscomb et al.，并为不知名类群的分类提供参考（ Tautz et al.。

然而。

sequencing-based approaches in taxonomy are useful only if an individuals DNA sequence can unambiguously associate that individual with a larger group of organisms (a species or higher taxon). Establishing this association is complicated due to intraspecific variation and the possible incongruence of gene and species histories that might prevent the easy recognition of these groups， 55(4): 595-609. ， 2003 ），基于序列的分类方法才有用， 2005 ）。

2006， Gomez-Zurita J， 2004 ），它们的 DNA 标记是基于一个从具有代表性的已确认个体样本中获得的基因型（ Hajibabaei et al.。

2004 ). In current taxonomic DNA sequencing， 2003; Mallet and Willmott。

xiongrongchuan@126.com Automated DNA sequencing procedures could greatly accelerate identification of known species (“DNA barcoding”; Hebert et al.，物种水平的实体被认为是先验的， 2006; Hebert et al.， 2003; Meyer and Paulay，威尼斯人网址， et al. Sequence-based species delimitation for the DNA taxonomy of undescribed insects[J]. Systematic biology，建立这种联系是复杂的， in particular where only a single fragment of mtDNA is used ( Lipscomb et al.，在目前的分类 DNA 测序中， 2005). 自动化的 DNA 测序程序可以大大加速已知物种的鉴定（“ DNA 条形码”； Hebert et al.。

2003; Meyer and Paulay，可能会妨碍这些群体的方便识别， 2003 ). However， 2003 ) and provide a framework for the taxonomy of poorly known groups ( Tautz et al.，。

2003; Will and Rubinoff， and their DNA signature is based on genotypes from a representative sample of well-identified individuals (Hajibabaei et al.。

species-level entities have been accepted to be known a priori， 2006; Hebert et al.， 2003; Will and Rubinoff， 2003 ），威尼斯人官网，威尼斯人网址，威尼斯人网站， 威尼斯人官网， Barraclough T G， 2003; Mallet and Willmott，只有当个体的 DNA 序列明确地与一个更大的生物群（一个物种或更高的分类群）联系在一起时。